Problem-based learning (PBL) method is designed to provide knowledge and skills demanded for a job requirement. The ability to continuously learn is needed in solving new problems and challenges, and thus an ever-evolving ability is absolutely needed. This study aims to reveal the effect of the PBL on students' critical thinking ability in entrepreneurship practice course at Economics Education Department of the College of Education and Teachers’ Training PGRI Jombang East Java Indonesia. It employed quantitative research. The data was collected by observation and questionair. The sample in this research was 32 college students majoring in Economics Education Department of 2015 batch at the College of Education and Teachers’ Training PGRIJombang. It applied simple linear regression test for data analysis. The finding showed that the significance of ttest and Ftest is less than Alpha coefficient, 0.000 <0.05. It implied that there is significant effect of PBL method on students' critical thinking ability on entrepreneurship practice course of Economics Education Department of the College of Education and Teachers’ Training PGRI Jombang East Java Indonesia.